Halsavit Tablets – Advanced Superfood for Energy & More!

Research

Note: Read the leaflet carefully before you start taking this alternative medicine and keep it safe in case you need to refer to it again for information regarding usage and dosage. If you have any further questions, consult your doctor or contact us through our website “www.trendspharmaceuticals.com”. If the symptoms worsen or persist, consult your healthcare physician.

This is a brief document and does not represent complete information regarding beneficial effects of Halsavit. The information shared is primarily focused on the use of Halsavit as a nutritional supplement with natural anti-viral, immuno-protective, and antioxidant effects. The given information in any case does not limit or understate health benefits associated with daily consumption of Halsavit.

HALSAVIT

Description:

Halsavit contains Spirulina, a clinically-researched blue-green algae. Widely hailed as the most nutritionally dense food on the planet, this wonder food has been praised for its significant health benefits as a dietary supplement. Enriched with a unique combination of phytonutrients, vitamins, minerals, amino acids and essential fatty acids, Halsavit is a powerful immunity booster, antioxidant, natural antiviral, anti-cancer, and anti-bacterial. It also enhances energy, lowers bad cholesterol, helps control blood pressure/diabetes, improves symptoms of allergic rhinitis and much more.

Product Form: Tablets & Syrup
Size: 30 Tablets & 120ml Syrup

Supplement Facts:

** Daily Value not established

Nutrient Profile of Halsavit

Mechanisms of Action:

Halsavit’s main ingredient, Spirulina (Arthrospira Platensis) is a unique freshwater plant that has been of an enormous interest to nutritional scientists over the past decade. Spirulina’s concentrate nutrition, as shown in Halsavits nutrient profile, makes it an ideal food supplement for people of all ages and lifestyles. It has a long history of human consumption. Currently Spirulina is used as a nutraceutical ingredient due to its unique combination of phytonutrients like phycocyanin, carotenes, xanthophylls, GLA, galactolipids, sulfolipids, chlorophyll and minerals. The three major bioactive components of Spirulina, biliprotein pigment Phycocyanin, sulfated polysaccharides and gamma linolenic acid play significant roles in improving human body functions. New experimental evidences support immunomodulation, potential health benefits and antiviral effects of Spirulina supplementation are mainly due to its chemical composition. In addition to Spirulina’s potential health effects, such as antibacterial, antioxidant, anticancer, immunomodulation and antiviral activities, some other positive effects were also observed against some disorders like malnutrition, diabetes, hyperlipidemia, inflammatory allergic reactions, obesity, anemia, heavy metal/chemical-induced toxicity and radiation damage.

Moreover, Halsavit’s second main ingredient, Ginkgo Biloba is one of the oldest living tree species, dating back over 300 million years, and individual trees can live for over 1,000 years. In China extracts of the fruit and leaves of the ginkgo tree have been used for over 5,000 years to treat lung ailments such as asthma and bronchitis, and as a remedy for cardiovascular diseases. Recently western researchers have been studying ginkgo biloba as a treatment for senility, hardening of the arteries, and as a treatment for oxygen deprivation. More than 34 human studies on ginkgo have been published since 1975, showing, among other things, that ginkgo can increases the body’s production of the universal energy molecule adenosine triphosphate, commonly called ATP. This activity has been shown to boost the brains energy metabolism of glucose and increase electrical activity.

Scientists also discovered that ginkgo contains an abundance of useful compounds including the antioxidants Vitamin C and carotenoids, but it is the flavonoid compounds collectively known as “ginkgolides” that are the most remarkable. The ginkgo flavonoids act specifically to dilate the smallest segment of the circulatory system, the micro-capillaries, which has a widespread affect on the organs, especially the brain. Researchers have also reported that Ginkgo extracts effectively increase blood circulation and increase oxygen levels in brain tissues. Ginkgo is also a powerful antioxidant that prevents platelet aggregation inside arterial walls, keeping them flexible and decreasing the formation of arteriosclerositic plaque.

Antiviral properties

Extracts from blue green algae, including Spirulina platensis, studied by researchers at the National Cancer Institute (NCI), USA yielded promising antiviral activity. The effect was mediated by a reduction in the cytopathic effects caused by the invading pathogen. Acidic polysaccharides such as calcium spirulan (Ca-SP) present in Spirulina platensis act against and inhibit the replication of several viruses. This potent and broad-spectrum antiviral activity particularly targeted enveloped viruses such as Influenza A, HIV, Herpes Simplex, Cytomegalovirus, Mumps and Measles Viruses. The cytopathic role of these viruses and replication was inhibited in human T cells, peripheral blood mononuclear cells and Langerhans cells where prevention of virus-cell attachment and fusion with host cells was observed. The Ca-SP also targeted viral absorption/penetration and some replication stages of progeny viruses. The water extract of Spirulina platensis interferes with the virus entry into host cells, particularly of HSV-1. Overall results showed that the Ca-SP, methanol (MeOH) and water extracts of Spirulina platensis were active in preventing replication of a host of enveloped viruses. These established mechanisms against RNA viruses such as SARS-CoV, Dengue, Influenza A, Mumps, Measles, Hepatitis C, HIV, all may indicate potential efficacy of spirulina platensis in a wide variety of coronaviruses as well and may even be beneficial for Covid-19.

Figure 1: Anti-viral properties of cyanobacterium, Spirulina Platensis. (2)

Immunomodulatory properties

Spirulina enhances components of the mucosal and systemic immune system by activating the cells of the innate immune system. The aqueous extract of spirulina was found to have a major impact on the immune system by increasing the phagocytic activity of macrophages and stimulating the NK cells. This also enhanced NK-mediated IFNγ production. It has also been noted that in in-vivo and in-vitro it suppressed the growth of tumor cells and induced the lymphocytes in the spleen to produce TNF-α. Due to its stimulatory effects in the production of cytokines and antibodies, it also mobilized the adaptive immune system and played a role in the activation and mobilization of T and B cells. The strong IL-1β, IL-responses elicit an age-dependent enhancement of the adaptive immune response. Spirulina was also effective in potentiating the cell-mediated immunity by stimulating the Th-1 type response. A cytokine array test was performed noted that spirulina had an increased effect on the expression of genes encoding the chemokines interleukin (IL)-8, IP-10, the cytokines TNF-α, IL-1β, and the enzyme cyclooxygenase-2 (COX-2). Moreover, the effects of phycocyanin (a biliprotein of Spirulina) is seen to enhance the biological defense activity by reducing allergic inflammation. This is carried out by suppression of antigen-specific IgE antibodies and through maintaining the mucosal immune system function against infectious diseases.

Figure 2: Immunomodulatory potentials of Spirulina in innate and adaptive immune responses along with its role in hypersensitive reactions.

Antioxidant properties

A phytochemical richly present in Spirulina, phycocyanobilin, mimics the potent inhibitory impact of biliverdin and free bilirubin on NADPH oxidase complex, thereby inhibiting its activity and promoting synthesis of increased antioxidant reduced glutathione (GSH) and reducing oxidized glutathione (GSSG). This decreases oxidative stress as well as concentrations of free radicals. Phycocyanin has been observed to play a major role in scavenging hydroxyl and peroxyl radicals. A double-blind, placebo-controlled study performed on individuals after exercise, showed decreased creatine kinase when they were supplemented with Spirulina. There was also a marked increase in exercise performance and GSH concentrations and a reduction in lipid peroxidation owing to the antioxidant properties of Spirulina. Protein extracts containing phycobiliproteins have been postulated to provide protection against teratogenesis secondary to reactive oxygen species by reducing oxidative stress. Protein extract and the biliprotein phycocyanin increase GSH in cells in order to reduce oxidative stress induced by iron. This was discovered by exposing human neuroblastoma cells in vitro to toxic amounts of iron and then to Spirulina. It was observed that Spirulina effectively protects the activity of the cellular antioxidant enzymes and reduces iron-induced oxidative stress as well as radical-mediated cell death. Geriatric patients who were administered Spirulina for 16 weeks showed a remarkable improvement in the antioxidant potential, as measured by the increased levels of antioxidant status in plasma of these individuals. Reversal of age-related declines in memory and learning were also observed.

Figure 3: Antioxidant properties of Spirulina and their mechanisms

Therapeutic Indications/Health benefits:

• Energy and Vitality Booster – Increased Power Output
  • 2g Spirulina daily for 8 weeks was associated with an increase in power output and mean power output but not with muscular endurance. (90)
  • 6g of spirulina for 4 weeks was associated with prolonged time to exhaustion in a 2-hour run secondary to increased fat oxidation, and also suppressed parameters of oxidation. (91)
• A natural Anti-viral that helps reduce viral load
  • This potent and broad-spectrum antiviral activity of Halsavit particularly targets RNA enveloped viruses such as Influenza A, HIV, Herpes Simplex, Cytomegalovirus, Mumps and Measles Viruses. Established mechanisms against RNA viruses such as, Dengue, Influenza A, Mumps, Measles, Hepatitis C, HIV, all indicates potential efficacy of spirulina platensis in a wide variety of coronaviruses and may be beneficial for Covid-19.
  • Spirulina, in Halsavit is capable of inducing a sustained virologic response in some persons with Hepatitis C, at about 20% of persons (13.3% complete, 6.7% with some degree of improvement) (87)
• A potent Antioxidant with free radical scavenging capabilities
  • 8g of Spirulina for 4 months was associated with improved lipid profile, immune variables, and antioxidant capacity; more-so in females, but favorable inflammatory changes in men. (89)

• Immunity booster with innate and adaptive immunomodulation properties
  • Two pilot studies, independent and their results combined, note increases of NK cell mRNA and subsequent NK cell tumor-killing activity with 200-400mg Spirulina a day. (86)
  • 3g Spirulina daily for 12 weeks in elderly persons with a history of anemia improved blood parameters (without influencing RBC) and increased WBC count. (88)
• Inflammatory Disorders:
  • It exhibits an anti-inflammatory activity by acting on the cyclo oxygenase enzyme 3, inhibiting production of prostaglandins and leucotrienes.
• Helps manage Type 2 Diabetes:
  • In a human study, Spirulina supplementation at 2 g/day for 2 months given to diabetic patients improved glycosylated hemoglobin (HbA1C) and favorably altered the serum lipid profile. These studies show that Spirulina can be used as a functional food to manage Type 2 Diabetes.
• Hypertension:
  • In humans, Spirulina reduced blood pressure in hypertensive subjects when given 4.5 g Spirulina for 6 weeks. Spirulina supplementation reduced both systolic and diastolic blood pressure in subjects.
• Cholesterol Reduction
  • Spirulina consumption reduces Serum cholesterol levels by 4.5% in 4 weeks.
  • In a human study, the effect of orally supplemented Spirulina (4.5 g/day, for 6 weeks) was evaluated in Mexican population. Spirulina significantly reduced serum triglyceride values and boosted HDL and lowered total cholesterol in the test subjects.
• Anti-Cancer Activity
  • Evaluation of chemo prevention of oral cancer with spirulina evaluated the chemo preventive activity of spirulina (1 gm./day for 12 months) in reversing oral leukoplakia in pan tobacco chewers in Kerala, India.
  • Complete regression of lesions was observed in 20 of 44 (45%) evaluated subjects supplemented with spirulina, as opposed to 3 of 43 (7%) in the placebo arm.
  • Certain special enzymes called “Endonucleases” repair damaged DNA to keep cells alive and healthy. When these enzymes are deactivated by toxins or radiation, errors in DNA go un-repaired and may eventually result in cancer. Spirulina prevents deactivation of Endonucleases, thereby preventing and even correcting early cancers.
  • Enhancement of endonuclease activity and repair DNA synthesis by polysaccharide of spirulina.
  • Exhibited inhibitive effect and mechanism of polysaccharide of spirulina on transplanted tumour cells in mice.
• Radiation protection
  • Ionizing radiation can cause mutations in the bone marrow cells. Spirulina polysaccharide was shown to reduce mutagenesis in animal studies.
• Lipid peroxidation Inhibition in arthritis, Alzheimer’s, immunological disorders:
  • Lipid peroxidation causes damage to cell membranes. Research suggests that phycocyanin present in spirulina inhibits lipid peroxidation at a concentration of 5oomM in rats microsomes
• Anti-platelet:
  • It inhibits platelet aggregation by reducing prostaglandin mediated thromboxane A2 formation, thus acting as a thromboxane synthase inhibitor.
• Neurodegenerative Disorders:
  • Neurodegenerative disorders like Parkinson’s and Alzheimer’s are associated with oxidative stress. Preclinical experiments with rats showed that phycocyanin had potential to prevent oxidative stress produced in the hippocampus of experimental animals.
• Age related Cognitive Decline:
  • Age related cognitive decline can be due to inflammation related oxidative damage to the central nervous system (CNS). Diet high in antioxidant activity is able to reduce this decline. Spirulina was shown to reverse age-induced decreases in cerebellar β-adrenergic function in rats, and this effect may be due to high antioxidant capacity of Spirulina.
• Neurogenesis:
  • Neurogenesis or production of new neurons is a lifelong occurrence, and necessary for learning. Inflammation causes a decrease in neurogenesis. In a rat model, diet enriched with Spirulina was able to negate this inflammation related decrease in brain neurogenesis.
• Neuroprotective:
  • Spirulina was shown to have neuroprotective effects against transient focal ischemia in rats. This effect could be due to the decrease in pro inflammatory cytokines with Spirulina diet.
• Kidney detoxification
  • Scientific studies suggest Spirulina may have a beneficial effect for humans suffering from heavy metal poisoning and that kidney side effects may decrease when spirulina is taken with the administration of drugs.

Who Should Consume Halsavit Daily?

Health conscious people:

• Halsavit is for those who want to just live and enjoy a healthy life.

Children:

• Children usually dislike vegetables. Nevertheless, they need a balanced diet to ensure proper growth and to prevent them from acquiring and nutritionally deficient diseases, which could be achieved by Halsavit.

Busy People:

• People who do not have time to keep track of their balanced diet. They will be supplied with all the necessary nutrients and regain their energy quickly.

Athletes and Sports people:

• They can enjoy long lasting energy and reduction in recovery time after exercise.

People who are recovering from illnesses:

• Will provide complete nourishment and quicken the healing process.

Weight Watchers:

• Halsavit contains phenylalanine which acts on the brain’s appetite center as a weight suppressant, free from the dangers of slimming pills.

People with Low Immunity:

• Low immunity increases the risk of developing viral and bacterial infections.

Type 2 Diabetes:

• Reducing systemic inflammation
• Favorably altering your lipid profile by reducing serum triglycerides and increasing HDL.
• Improving vasodilation in those who are obese as a result of high fructose diets (which has benefits for diabetics, as well as for those with hypertension and cardiovascular disease)

Cataract patients:

• Reducing age-related macular degeneration (ARMD) i.e deterioration of your macula (the region in your eye that controls acute vision)
• Spirulina provides carotenoid pigments called xanthophylls—lutein, zeaxanthin, and possibly astaxanthin which help protect the eyes from damage by slowing down ultraviolet-induced oxidation of lipid membranes, thereby helping prevent degeneration of the macula.

Heart patients:

• Role of Spirulina in terms of creating better lipid profiles, controlling hypertension, and increasing blood vessel elasticity
• Decreases in blood pressure and plasma lipid concentrations, especially triacylglycerols and low-density lipoprotein-cholesterol have been demonstrated as a result of oral consumption of Spirulina.
• Results also showed that HDL levels saw a significant increase, while both systolic and diastolic blood pressure decreased. Improved glycosylated hemoglobin and prevented hypertension and vasoconstriction.

Suffering from erythromycin induced liver toxicity:

• Protective agent as lipid peroxidation reduced with increase in hepato-protective glutathione.

Suffering from Non-alcoholic fatty liver disease:

• Prevents the buildup of triglycerides in your liver
• Inhibits lipid peroxidation
• Reduces liver inflammation
• Protects your liver from damage by heavy metals, like lead and mercury.

 Prevention of Stroke

• Reduces platelet aggregation, reducing risk for thromboembolism.
• Highest neuroprotective effect.
• Halsavit can suppress NADPH oxidase, lowering your risk for neurodegenerative disorders such as Parkinson’s and Alzheimer’s.

Anemic Patients:

Spirulina stimulates production of stem cells by bone marrow.

Suffering from arthritis:

Gamma linoleic acid manages inflammation and prevents arthritis.

Renal Failure:

Applicable to the management of general renal dysfunction and heavy metal poisoning.

Side effects and adverse effects:

The Dietary Supplements Information Expert Committee of the United States Pharmacopeia reviewed the safety of spirulina in 2011. The committee unanimously voted for a Class A safety assignment for spirulina, meaning that available evidence did not indicate it to pose a serious health risk when properly formulated and used.

In spite of the wide usage for the beneficial effects, a few side effects and contraindications have been reported with spirulina use. The most frequently reported adverse effects are headache, muscle pain, flushing of the face, sweating, and difficulty concentrating. These have been described in people taking 2 tablets or 20ml of Halsavit. Skin reactions have also been reported. Spirulina should be avoided by people who have the metabolic disorder phenylketonuria, as phenylalanine content can be harmful.

Drug Interactions:

Dosage and administration:

Oral route the tablets should be swallowed, without chewing, with half a glass of water at the end of a meal.

Syrup can be taken at the end of a meal.

Two (2) to four (4) tablets twice daily or Two (2) to four (4) tablespoon twice daily.

The specific doses depend on the condition its being used for:

• For immunity, four (4) tablets or 40 ml of syrup daily
• For cholesterol, doses in the range of two (2) tablets or 20 ml g per day may be impactful
• For energy and muscle performance, four (4) tablets or 40 ml of syrup daily have been used
• For blood glucose control, four (4) tablets or 40 ml of syrup daily
• Blood pressure may be affected at doses of four (4) tablets or 40 ml of syrup daily
• Effects for fatty liver have been seen at doses of 4.5 g per day

Overdosage:

Up to five (5) tablets or 50 ml of Halsavit daily is completely safe according to research. Adverse symptoms may occur if consumption is increased beyond the safe limit.

Inform your doctor immediately in case of massive absorption.

Shelf life:

Do not use after two (2) years starting from the date of manufacturing or as per the expiry date mentioned on the box.

Storage conditions:

• Store below 30 C.
• Keep in a dry place
• Keep away from direct sunlight and heat
• Keep all medications out of the reach and sight of children

Manufactured by:

Trends Pharmaceuticals

References:

1. Ciferri O (1983) Spirulina, the edible microorganism Microbiol Rev 47(4) 551-578
2. ANTIVIRAL PROPERTIES OF CYANOBACTERIUM, SPIRULINA PLATENSIS-A REVIEW International Journal of Medicine and Pharmaceutical Sciences (IJMPS) ISSN(P): 2250-0049; ISSN(E): 2321-0095 Vol. 3, Issue 5, Dec 2013, 1-10.
3. The beneficial effects of spirulina focusing on its immunomodulatory and antioxidant properties – Nutrition and Dietary Supplements 2010:2 73–83
4. Mittal A, Kumar PV, Banerjee S, Rao AR, Kumar A. Modulatory potential of Spirulina fusiformis on carcinogen metabolizing enzymes in Swiss albino mice. Phytother Res. 1999;13(2):111–114.
5. Mao TK, Van de Water J, Gershwin ME. Effects of a Spirulina-Based Dietary Supplement on Cytokine Production from Allergic Rhinitis Patients. J Med Food. 2005;8(1):27–30.
6. Sammon AM. Dietary Linoleic acid, immune inhibition and disease. Postgrad Med J. 1999;75(881):129–132.
7. Kulshreshtha A, Zacharia J, Jarouliya U, Bhadauriya P, Prasad GBKS, Bisen PS. Spirulina in Health Care Management. Current Pharm Biotechnol. 2008;9(5):400–405.
8. Watanabe H, Pan ZQ, Schreiber-Agus N, DePinho RA, Hurwitz J, Xiong Y. Suppression of cell transformation by the cyclin-dependent kinase inhibitor p57KIP2 requires binding to proliferating cell nuclear antigen. Proc Natl Acad Sci U S A. 1998;95(4):1392–1397.
9. Chen T, Wong YS, Zheng W. Induction of G1 cell cycle arrest and mitochondria-mediated apoptosis in MCF-7 human breast carcinoma cells by selenium-enriched Spirulina extract. Biomed Pharmacother. 2009;27. Epub ahead of print 2009.
10. Dasgupta T, Banejee S, Yadav PK, Rao AR. Chemomodulation of carcinogen metabolising enzymes, antioxidant profiles and skin and forestomach papillomagenesis by Spirulina platensis. Mol Cell Biochem. 2001;226(1–2):27–38.
11. MishimaT,MurataJ,ToyoshimaM,etal.Inhibitionoftumorinvasion and metastasis by calcium spirulan (Ca-SP), a novel sulfated polysac- charide derived from a blue-green alga, Spirulina platensis. Clin Exp Metastasis. 1998;16(6):541–550.
12. AkaoY,EbiharaT,MasudaH,etal.Enhancementofantitumornatural killer cell activation by orally administered Spirulina extract in mice. Cancer Sci. 2009;100 (8):1494–501. Epub 2009 May 6.
13. Chen T, Wong YS. In vitro antioxidant and antiproliferative activities of selenium-containing phycocyanin from selenium-enriched Spirulina platensis. J Agric Food Chem. 2008 25;56(12):4352–4358. Epub 2008 Jun 4.
14. Hayashi T, Hayashi K, Maeda M, Kojima I. Calcium spirulan, an inhibi- tor of enveloped virus replication, from a blue-green alga Spirulina platensis. J Nat Prod. 1996;59(1):83–87.
15. Ayehunie S, Belay A, Baba TW, Ruprecht RM. Inhibition of HIV-1 tensis (Arthrospira platensis). JAIDS: J Acquir Immune Def Synd Human Retrovirol. 1998;18(11):7–12.
16. Hayashi T, Hayashi K, Maeda M, Kojima IA. Natural sulfated polysaccharide, calcium spirulan, isolated from Spirulina platen- sis: in vitro and ex vivo evaluation of anti-herpes simplex virus and anti-human immunodeficiency virus activities. AIDS Res Hum Retroviruses.1996;12(15):1463–1471.
17. Simpore J, Zongo F, Kabore F, Dansou D, Bere A, Nikiema JB. Nutrition Rehabilitation of HIV-Infected and HIV-Negative Under- nourished Children Utilizing Spirulina”. Annal Nut Metabolism. 2005;49(6):373–380.
18. Park HJ, Lee YJ, Ryu HK, Kim MH, Chung HW, Kim WYA. Random- ized double-blind, placebo-controlled study to establish the effects of Spirulina in elderly Koreans. Annal Nutr Metabol. 2008;52(4): 322–329.
19. Khan Z, Bhadouria P, Bisen PS. Nutritional and therapeutic potential of spirulina. Curr Pharm Biotechnol. 2005;6(5):373–379.
20. Lee AN, Werth VP. Activation of Autoimmunity Following Use of Immunostimulatory Herbal Supplements. Arch Dermatol. 2004;140(6): 723–727.
21. Zhang HQ, Lin AP, Sun Y. Chemo and radio protective effects of poly- saccharide of spirulina platensis on haematopoietic stem cells in dog and mice. Acta Pharmacol Sin. 2001;22(12):1121–1124.
22. Remirez D, Ledon N, Gonzale R. Role of histamine in the inhibitory effects of phycocyanin in experimental models of allergic inflammatory response. Med Inflamm. 2002;11(2):81–85.
23. Hayashi O, Katayanagi Y, Ishii K, Kato T. Flow cytometric analysis of age-related changes in intestine intraepithelial lymphocyte subsets and their functional preservation after feeding mice on spirulina. J Med Food. 2009;12(5):982–989.
24. Rasool M, Sabina EP. Appraisal of immunomodulatory potential of Spirulina fusiformis: an in vivo and in vitro study. J Nat Med. 2009; 63(2):169–175.
25. Løbner M, Walsted A, Larsen R, Bendtzen K, Nielsen CH. Enhance- ment of human adaptive immune responses by administration of a high-molecular-weight polysaccharide extract from the cyanobacterium Arthrospira platensis. J Med Food. 2008;11(2):313–322.
26. Trushina EN, Gladkikh O, Gadzhieva ZM, Mustafina OK, Pozdniakov AL. The influence of Spirulina and Selen-Spirulina on some indexes of rat’s immune status Article Russian Vopr Pitan. 2007;76(2):21–25.
27. Balachandran P, Pugh ND, Ma G, Pasco DS. Toll-like receptor 2-dependent activation of monocytes by Spirulina polysaccharide and its immune enhancing action in mice. Int Immunopharmacol. 2006;6(12): 1808–1814.
28. Grzanna R, Polotsky A, Phan PV, Pugh N, Pasco D, Frondoza CG. Immolina, a high-molecular-weight polysaccharide fraction of Spirulina, enhances chemokine expression in human monocytic THP-1 cells. J Altern Complement Med. 2006;12(5):429–435.
29. Pugh N, Ross SA, ElSohly HN, ElSohly MA, Pasco DS. Isola- tion of three high molecular weight polysaccharide preparations with potent immunostimulatory activity from Spirulina platensis, aphanizomenon flos-aquae and Chlorella pyrenoidosa. Planta Med. 2001;67(8):737–742.
30. Nemoto-Kawamura C, Hirahashi T, Nagai T, Yamada H, Katoh T, Hayashi O. Phycocyanin enhances secretary IgA antibody response and suppresses allergic IgE antibody response in mice immunized with antigen-entrapped biodegradable microparticles. J Nutr Sci Vitaminol (Tokyo). 2004;50(2):129–136.
31. Cingi C, Conk-Dalay M, Cakli H, Bal C. The effects of Spirulina on allergic rhinitis. Eur Arch Oto-Rhino-Larynol. 2008;265(10): 1219–1223.
32. Hirahashi T, Matsumoto M, Hazeki K, Saeki Y, Ui M, Seya T. Activa- tion of the human innate immune system by Spirulina: augmentation of interferon production and NK cytotoxicity by oral administration of hot water extract of Spirulina platensis. Int Immunopharmacol. 2002;2(4):423–434.
33. Qureshi MA, Garlich JD, Kidd MT. Dietary Spirulina platensis enhances humoral and cellmediated immune functions in chickens. Immuno- pharmacol Immunotoxicol. 1996;18(3):465–476.
34. Hayashi O, Katoh T, Okuwaki Y. Enhancement of antibody production in mice by dietary Spirulina platensis. J Nutr Sci Vitaminol (Tokyo). 1994;40(5):431–441.
35. McCarty MF. Practical prevention of cardiac remodeling and atrial fibrillation with full-spectrum antioxidant therapy and ancillary strate- gies. Med Hypoth. 2010.
36. McCarty MF. Potential utility of full-spectrum antioxidant therapy, citrulline, and dietary nitrate in the management of sickle cell disease. Med Hypoth. Epub 2010 Jan 18.
37. Mendiola JA, Martín-Alvarez PJ, Señoráns FJ, et al. Design of natural food antioxidant ingredients through a chemometric approach. J Agric Food Chem. 2010;58(2):787–792.
38. Barmejo-Bescós P, Piñero-Estrada E, Villar DF, A. Neuroprotection by Spirulina platensis protean extract and phycocyanin

against iron- induced toxicity in SH-SY5Y neuroblastoma cells. Toxicol in Vitro. 2002;22(6):1496–1502.

39. Lu HK, Hsieh CC, Hsu JJ, Yang YK, Chou HN. Preventative effects of Spirulina platensis on skeletal muscle damage under exercise induced oxidative stress. Eur J Appl Physiol. 2006;98(2):220–226.
40. Kalafati M, Jamurtas AZ, Nikolaidis MG, et al. Ergogenic and antioxi- dant effects of spirulina supplementation in humans. Med Sci Sports Exerc. 2010;42(1):142–151.
41. Vázquez-Sánchez J, Ramón-Gallegos E, Mojica-Villegas A, et al. Spirulina maxima and its protein extract protect against hydroxyu- rea-teratogenic insult in mice. Food Chem Toxicol. 2009;47(11): 2785–2789.
42. Bedirli A, Kerem M, Ofluoglu E, et al. Administration of Chlorella sp. microalgae reduces endotoxemia, intestinal oxidative stress and bacterial translocation in experimental biliary obstruction. Clin Nutr. 2009;28(6):674–678.
43. Gemma C, Mesches MH, Sepesi B, Choo K, Holmes DB, Bickford PC. Diets enriched in foods with high antioxidant activity reverse age- induced decreases in cerebellar beta-adrenergic function and increases in proinflammatory cytokines. Experiment Neurol. 2002;22(14): 6114–6120.
44. Robb-Nicholson C. By the way, doctor. Harvard Women’s Health Watch 8.2006.
45. Li B, Gao MH, Zhang XC, Chu XM. Molecular immune mechanism of C-phycocyanin from Spirulina platensis induces apoptosis in HeLa cells in vitro. Biotechnol Appl Biochem. 2006 Mar;43(Pt 3):155–164.
46. Armida Zúñiga-Estrada, Blanca Lilia Barrón, J Martín Torres-Valencia, & Germán Chamorro- Cevallos (2007).Spirulina as an Antiviral Agent, 227-242.
47. Abdulmumin A. Nuhu (2013). Spirulina (Arthrospira): An Important Source of Nutritional and Medicinal Compounds, Journal of Marine Biology, Volume 2013, Article ID 325636, 8.
48. Amha Belay (2002), The potential applications of Spirulina (Arthrospira) as a nutritional and therapeutic supplement. J. American. Neutraceutical Association (Spring, 2002), 5(2), 27-48.
49. Archana Kulshestha, Anish Zacharia, J., Urmila Jarouliya, Pratiksha Bhadauriya, Prasad.G.B. K.S. & Bisen, P.S. (2008). Spirulina in health care management, Curr. Pharm. Biotechnol, 9 (5): 400-405.
50. Ayehunie S, Belay A, Baba T.W & Ruprecht R.M. (1998). Inhibition of HIV-1 replication by an aqueous extract of Spirulina platensis (Arthrospira platensis). J Acquired Immune Deficiency Syndromes and Human Retrovirology.18:7-12.
51. Blinkova, L.P, Gorobets, O.B & Baturo, A.P (2001). Biological activity of Spirulina, Zh Mikrobiol Epidemiol Immunobiol, 2, 114 -118.
52. Ayehunie S, Belay A, Baba TW, Ruprecht RM. Inhibition of HIV-1 tensis (Arthrospira platensis). JAIDS: J Acquir Immune Def Synd Human Retrovirol. 1998;18(11):7–12.
53. Hayashi T, Hayashi K, Maeda M, Kojima IA. Natural sulfated polysaccharide, calcium spirulan, isolated from Spirulina platen- sis: in vitro and ex vivo evaluation of anti-herpes simplex virus and anti-human immunodeficiency virus activities. AIDS Res Hum Retroviruses.1996;12(15):1463–1471.
54. Capelli, B. & Cysewski, G. R. (2010) Nutrafoods 9, 19.
55. Chirasuwan Nattayaporn, Ratana Chaiklahan, Prasat Kittakoop, Wanlop Chanasattru, Marasri Ruengjitchatchawalya, Morakot Tanticharoen & Boosya Bunnag (2009). Anti HSV-1 activity of sulphoquinovosyl diacylglycerol isolated from Spirulina platensis, ScienceAsia 35: 137–141.
56. Chirasuwan, N, Chaiklahan, R, Ruengjitchatchawalya, M, Bunnag, B, & Tanticharoen, M. (2007) Anti HSV-1 activity of Spirulina platensis polysaccharide. Kasetsart J (Nat Sci) 41, 311–8.
57. von der Weid (2000). Malnutrition: a silent massacre, Antenna Technologies, http://www.antenna.ch/en/documents/MonoUK1.pdf (accessed on 7/12/2012).
58. Belay, A. (2008) Spirulina in Human Nutrition and Health, M. E. Gershwin, A. Belay, Eds.,CRC Press, Taylor and Francis Group, Boca Raton, London, New York. p. 1.
59. Juarez MA, Mascher D, Torres PV, Farias JM. Effects of spirulina on vascular reactivity. J Med Food. 2009;12(1):15–20.
60. Lee AN, Werth VP. Activation of Autoimmunity Following Use of Immunostimulatory Herbal Supplements. Arch Dermatol. 2004;140(6): 723–727.
61. Gorobets, O.B, Blinkova, L.P & Baturo, A.P. (2002). Action of Spirulina platensis on bacterial viruses, Zh Mikrobiol Epidemiol Immunobiol. (6):18-21.
62. Gustafson, K.R, Cardellina, J.H II, Fuller, R.W, WeslowO.S, Kiser, R.F, Snader, K.M, Paterson, G.M, & Boyd, M.R (1989). AIDS-antiviral sulfolipids from cyanobacteria (blue green algae). J Natl Cancer Inst81: 1254-1258.
63. Hayashi K, Hayashi T, & Kojima I. (1996). A natural sulfated polysaccharide, calcium spirulan, isolated from Spirulina platensis: in vitro and ex vivo evaluation of anti-Herpes simplex virus and anti-human immunodeficiency virus activities. AIDS Research and Human Retroviruses, 12:1463-1471.
64. Shklar G, Schwartz J. Tumor necrosis factor in experimental cancer regression with alphatocopherol, beta-carotene, canthaxanthin and algae extract. Eur J Cancer Clin Oncol. 1988 (5):839–850.
65. Hayashi, K. Hayashi, T & Morita, N. (1993). An extract from Spirulina platensis is a selective inhibitor of Herpes simplex virus type 1 penetration into HeLa cells. Phytother Res.,7:76-80.
66. Hayashi, T., Hayashi, K Maedaa M, & Kojima, I. (1996). Calcium spirulan, an inhibitor of enveloped virus replication, from a bluegreen alga Spirulina platensis. J Nat Prod.59:83-87.
67. Hayashi T. (2008). Studies on evaluation of natural products for antiviral effects and their applications, Yakugaku Zasshi. 128(1):61-79.
68. Hemtanon, P., Direkbusarakom, S., Bunyawiwat, V. & Tantitakoon. O. (2005). Antiviral and Antibacterial Substances from Spiriluna platensis to combat White Spot Syndrome Virus and Vibrio harveyi. In P. Walker, R. Lester and M.G. Bondad- Reantaso (eds). Diseases in Asian Aquaculture V, pp. 525-534. Fish Health Section, Asian Fisheries Society, Manila.
69. Hernández-Corona A, Nieves I, Meckes M, Chamorro G, & Barron BL. (2002). Antiviral activity of Spirulina maxima against herpes simplex virus type 2. Antiviral Res.56 (3):279-85.
70. Kok, Y.Y, Chu, W. L, Phang, S.M, Mohamed, S.M, Naidu, R, Lai, P.J, Ling, S.N, Mak, J.W, Lim, P.K, Balraj, P & Khoo, A.S. (2011). Inhibitory activities of microalgal extracts against Epstein – Barr virus DNA release from lymphoblastoid cells, Zhejiang. Univ.Sci B, 12 (5), 335-45.
71. Ku, C.S, Pham, T.X, Park, Y, Kim, B, Shin, M.S, Kang, I & Lee, J (2013). Edible blue-green algae reduce the production of pro-inflammatory cytokines by inhibiting NF-kappaB pathway in macrophages and splenocytes. Biochim Biophys Acta, 1830(4):2981–2988.
72. Lau, A.F., Sied leck J, Anleitner J, Patterson GM, Caplan FR, & Moore RE (1993). Inhibition of reverse transcriptase activity by extracts of cultured blue green algae (cyanophyta), Planta Med., 59: 148 -51.
73. Lee, J.B, Srisomporn.P, Hayashi.K, Tanaka.T, Sankawa. U & Hayashi,T. (2001).Effects of structural modification of calcium spirulina, a sulphated polysaccharides from Spirulina platensis, on antiviral activity, Chem Pharm Bull (Tokyo), 49 (1), 108 – 110.
74. Mostafa Yakoot & Amel Salem (2012).Spirulina platensis versus silymarin in the treatment of chronic hepatitis C virus infection.A pilot randomized, comparative clinical trial BMC Gastroenterology, 12:32.
75. Khan M, Shobha JC, Mohan IK, Naidu MU, Sundaram C, Singh S. Protective effect of Spirulina against doxorubicin-induced cardio- toxicity. Phytother Res. 2005;19(12):1030–1037.
76. Patterson GML, Baker KK, Baldwin CL, Bolis CM, Caplan FR, Larson LK, Levine IA, Moore RE, Nelson CS, Tschappat KD & Tuang. G. (1993). Antiviral activity of cultured blue-green algae (Cyanophyta). J Phycol.29:125-130.
77. Sayda M. Abda, Mona H. Hetta, Waleed M. EI- Senousy, Rawheya, A. Salah EI Din & Gamila H. Ali (2012). Antiviral activity of fresh water algae, J. Applied pharmaceutical sciences, 2 (2), 21-25.
78. Shih, S.R, Tsai, K.N, Li,Y.S, Chueh,C.C & Chan, E.C (2003). Inhibition of enterovirus 71 – induced apoptosis by allophycocyanin isolated from a blue – green alga Spirulina platensis, J.Med. Virol., 70 (1), 119 – 25.
79. Witvrouw M & De Clercq E (1997) Sulfated polysaccharides extracted from sea algae as potential antiviral drugs. Gen Pharmacol 29:247–511.
80. Raffaella R, Marzia BG, Laura P, Elena M. Role of the p53/p21 system in the response of human colon carcinoma cells to Doxorubicin. Int J Bio Sci. 2009;5(4):377–387.
81. Schwartz J, Shklar G. Regression of experimental hamster cancer by beta carotene and algae extracts. J Oral Maxillofac Surg. 1987 Jun;45(6): 510–515.
82. Sheahan S, Bellamy CO, Harland SN, et al. TGF beta induces apoptosis and EMT in primary mouse hepatocytes independently of p53, p21Cip1 or Rb status. BMC Cancer. 2008;8:191–201.
83. Mendiola JA, Martín-Alvarez PJ, Señoráns FJ, et al. Design of natural food antioxidant ingredients through a chemometric approach. J Agric Food Chem. 2010;58(2):787–792.
84. Barmejo-Bescós P, Piñero-Estrada E, Villar DF, A. Neuroprotection by Spirulina platensis protean extract and phycocyanin against iron- induced toxicity in SH-SY5Y neuroblastoma cells. Toxicol in Vitro. 2002;22(6):1496–1502.
85. Vázquez-Sánchez J, Ramón-Gallegos E, Mojica-Villegas A, et al. Spirulina maxima and its protein extract protect against hydroxyu- rea-teratogenic insult in mice. Food Chem Toxicol. 2009;47(11): 2785–2789.
86. Enhancement of Natural Killer Cell Activity in Healthy Subjects by Immulina®, a Spirulina Extract Enriched for Braun-type Lipoproteins – Planta Med. 2010 Nov;76(16):1802-8.doi: 10.1055/s-0030-1250043. Epub 2010 Jun 17.
87. Spirulina Platensis Versus Silymarin in the Treatment of Chronic Hepatitis C Virus Infection. A Pilot Randomized, Comparative Clinical Trial – BMC Gastroenterol. 2012 Apr 12;12:32.doi: 10.1186/1471-230X-12-32.
88. The Effects of Spirulina on Anemia and Immune Function in Senior Citizens – Cell Mol Immunol. 2011 May;8(3):248-54. doi: 10.1038/cmi.2010.76. Epub 2011 Jan 31.
89. A Randomized Double-Blind, Placebo-Controlled Study to Establish the Effects of Spirulina in Elderly Koreans – Ann Nutr Metab. 2008;52(4):322-8. doi: 10.1159/000151486. Epub 2008 Aug 19.
90. Efficacy of spirulina supplementation on isometric strength and isometric endurance of quadriceps in trained and untrained individuals – a comparative study – JS Sandhu, Bhardwaj Dheera, Shenoy Shweta
    Department of Sports Medicine and Physiotherapy, Guru Nanak Dev University, Amritsar-143005, Punjab, India
91. Ergogenic and Antioxidant Effects of Spirulina Supplementation in Humans – Med Sci Sports Exerc. 2010 Jan;42(1):142-51.doi: 10.1249/MSS.0b013e3181ac7a45.